The quality and safety of ReoCell products are achieved through an innovative biotechnology laboratory equipped with cutting-edge technologies. This allows us to:
Processing refers to a set of technological procedures applied to cells from the moment they arrive in the laboratory until the final cell product is obtained for therapeutic or scientific use. Below are the main categories of equipment utilized in the ReoCell laboratory for processing:
This comprehensive laboratory setup ensures the safety of personnel and high-quality handling of cell materials.
Equipped with tools for efficient isolation, cultivation, and monitoring of cell growth conditions.
Outfitted with a tangential flow filtration system and an ultracentrifuge, along with equipment for counting and characterizing extracellular vesicles.
Fitted with a hollow fiber bioreactor that enables the large-scale accumulation of extracellular vesicles.
The Flow Cytometry Laboratory is designed for analyzing the physical and chemical properties of cells or particles in a liquid flow using a specialized instrument called a flow cytometer. This method is employed for detailed examination of cell populations and their characteristics, such as size, structure, DNA content, protein expression levels, and other biomarkers.
Analyze cells based on their physical (size, granularity) and chemical (fluorescent labeling) properties.
Facilitate the identification and quantitative analysis of various cellular markers.
The Biochemical Research Laboratory focuses on studying the chemical processes that occur during cell cultivation in laboratory conditions, as well as analyzing biomolecules and their interactions. The results obtained help to understand the mechanisms underlying the functioning of cultured cells.
This laboratory is essential for addressing a range of tasks related to the investigation of metabolic processes, drug development, and the exploration of fundamental aspects of cell biochemistry and extracellular microvesicles.
Analyzing the content of various substances, such as glucose, enzymes, proteins, lipids, and hormones in biological fluids and cell culture media, allows for the detection of functional disturbances or the presence of diseases.
Investigating metabolic processes, including carbohydrate, fat, protein, and nucleic acid metabolism, enhances our understanding of how cells and organisms obtain and transform energy to sustain life functions and perform specific tasks. This is crucial for developing treatment methods for metabolic diseases such as obesity, metabolic syndrome, and hereditary metabolic disorders.
Studying the interactions of pharmaceutical substances with cells, proteins, and enzymes, as well as their metabolism in the body, is an integral part of the drug development and testing process. This helps assess both the efficacy and safety of drugs, as well as their pharmacokinetics—from absorption to excretion.
Investigating enzymes that catalyze biochemical reactions within cells helps to understand how various biological processes are regulated and what disruptions can lead to diseases. Examining the structure and function of proteins allows for the development of new therapeutic methods, such as targeted therapies and enzyme replacement therapy.
To understand hereditary diseases, develop genetic therapy methods, and create diagnostic tests, the biochemical laboratory employs molecular biology techniques, including the study of nucleic acids (DNA and RNA), gene expression, and protein synthesis.
The Biochemical Research Laboratory investigates hormone levels in the media used for cell cultivation and their impact on various cultivation conditions.
Measure the concentration of substances in solutions by analyzing light absorption at specific wavelengths.
Separate and analyze components of complex mixtures.
Distribute proteins, nucleic acids, and other macromolecules based on their size and charge.
Provide rapid and accurate analysis of a large number of samples for various substances (enzymes, electrolytes, glucose, etc.).
The PCR Diagnostics Laboratory specializes in the detection and analysis of genetic material (DNA or RNA) in biological samples using the polymerase chain reaction (PCR) method. This technique is one of the most accurate and sensitive for diagnosing a variety of diseases, including infectious diseases, genetic disorders, and oncological processes. It allows for the detection of even minimal amounts of viruses or bacteria, making it indispensable for precise diagnostics and early disease detection.
The method helps detect pathogens (viruses, bacteria, fungi, parasites) in biological fluid samples.
Viral hepatitis B and C, as well as HIV, are often diagnosed using PCR, which allows not only for the detection of the virus but also for quantifying its amount (viral load).
In microbiology, PCR enables the identification of the infectious agent’s species and its strains, as well as assessing antibiotic resistance.
Facilitate the cyclical temperature changes necessary for the amplification (replication) of genetic material; may include a “Real-Time” function for quantitative PCR.
Prevent cross-contamination.
Allow for the amplification and measurement of DNA or RNA quantities in real time.
Automate the process of isolating DNA or RNA from samples, expediting and simplifying sample preparation for analysis.
Evaluate the quality and concentration of isolated nucleic acids prior to amplification.
The electron microscopy method allows for the acquisition of highly detailed images of objects using a beam of electrons, significantly surpassing the capabilities of conventional light microscopes.
The Electron Microscopy Laboratory is designed for studying the structures of materials and biological specimens at the nanoscale, with a primary focus on investigating extracellular microvesicles.
Investigating cells, tissues, viruses, bacteria, and organelles at the nanoscale allows for a deeper understanding of their functions and the identification of potential pathological changes. For example, the structure of cell membranes, mitochondria, ribosomes, and other critical cellular components can be examined.
Studying and characterizing nanoscale objects and extracellular vesicles helps identify structural defects, contributing to improved product quality.
Researching the interactions between biological tissues and various materials enhances our understanding of biocompatibility, aiding in the development of medical devices, implants, and other carriers.
Prepares ultrathin sections of biological or material samples.
Examines the internal structure of objects. TEM transmits a beam of electrons through a thin section of the sample, creating images of its internal structure with atomic-level resolution.
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